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Preservation of Bull Semen at Sub-Zero Temperatures   By:

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TRANSCRIBER'S NOTES: Transcription used in this e text: text indicates italics; =text= indicates bold face text; ^{text} indicates superscript text; {text} indicates subscript text. Lettered footnotes (with anchors [A], [B], etc.), explaining the text, have been moved to directly below the paragraph or table they refer to. Footnotes with anchors [1], [2], etc. refer to references, that are listed towards the end of the text. The original text has not been modified, except that some minor typographical errors have been corrected silently.

PRESERVATION OF BULL SEMEN AT SUB ZERO TEMPERATURES

By N. L. VanDemark W. J. Miller W. C. Kinney, Jr. Carlos Rodriguez M. E. Friedman

Bulletin 621

UNIVERSITY OF ILLINOIS AGRICULTURAL EXPERIMENT STATION

CONTENTS

Page

EARLY WORK ON FREEZING SEMEN 5

SELECTION OF SEMEN FOR FREEZING 6 Predicting freezability 6 Freezability of first and second ejaculates 7 Freezability of several consecutive ejaculates 7 Freezability of epididymal sperm 9 Freezability of washed sperm 10

EXTENDERS FOR FREEZING BULL SEMEN 10 Proportion of egg yolk in final diluent 10 Citrate level in final diluent 11 Storing and freezing diluent 12 Other diluents 13

DILUTION RATES 14 Effect of further dilution and refreezing 14

GLYCEROL ADDITIONS 17 Effect of glycerol on sperm survival at 5° C. 17 Glycerol levels for freezing semen 18 Rate, temperature, and method of adding glycerol 18 Allowing sperm to equilibrate with glycerol 20 Sugar additions and equilibration time 20 Substitutes for glycerol 22

FREEZING RATE 22 Effect of freezing rate on sperm survival 22 Rate of cooling in plastic and in glass 23

STORAGE TEMPERATURE 24 Storage at 23° to 79° C. 24 Use of higher glycerol levels and storage at 20° C. 25

THAWING 26 Comparison of thawing at 5° C. and at 38° C. 27 Thawing rate in plastic and in glass 29

EFFECTS OF FREEZING PROCEDURES ON METABOLIC ACTIVITY OF SPERM 30 Effect of glycerol additions on oxygen uptake of diluted semen at 37° C. 30 Effect of glycerol plus catalase on oxygen uptake of diluted semen 32 Effect of freezing procedures on oxygen utilization 33 Effect of freezing procedures on methylene blue reduction time 33

PRACTICAL FREEZING PROCEDURE 35 Collection of semen 35 Preparation of extender 35 Dilution after collection 36 Adding the glycerol 36 Equilibration 36 Freezing 37 Thawing 37

LITERATURE CITED 38

TEMPERATURE CONVERSION TABLE 39

Urbana, Illinois October, 1957

Publications in the Bulletin series report the results of investigations made or sponsored by the Experiment Station

ACKNOWLEDGMENT

The authors gratefully acknowledge the financial assistance given to the Department of Dairy Science in support of these investigations by the Southern Illinois Breeding Association of Breese, Illinois, and the Northern Illinois Breeding Co op of Hampshire, Illinois, through the Illinois Dairy Breeding Federation... Continue reading book >>




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